Sensitive nerve endings8/8/2023 ![]() ![]() The capsaicin receptor: a heat-activated ion channel in the pain pathway. Posttranslational mechanisms of peripheral sensitization. Voltage-gated sodium channels and pain pathways. Capsaicin-induced ion fluxes in dorsal root ganglion cells in culture. Contribution of Na(v)1.8 sodium channels to action potential electrogenesis in DRG neurons. A novel heat-activated current in nociceptive neurons and its sensitization by bradykinin. Distinct mechanosensitive properties of capsaicin-sensitive and -insensitive sensory neurons. A Bibliography of the Research in Tissue Culture (Academic Press, New York, 1953).ĭrew, L.J., Wood, J.N. ![]() Dissociated dorsal root ganglia in tissue culture. In addition, stimulation techniques, protocols to achieve single-fiber recordings, issues of data acquisition and action potential discrimination are discussed in detail. We describe the components and the setting-up of the basic equipment of a skin–nerve recording station (few days), the preparation of the skin and the adherent saphenous nerve in the mouse (15–45 min) and the isolation and recording of neurons (approximately 1–3 h per recording). ![]() Responses recorded from single-fibers are comparable with those obtained in previous in vivo experiments on the same species. Cutaneous nerve endings show graded sensitivities to various stimulus modalities that are quantified by adequate and controlled stimulation of the superfused skin with heat, cold, touch, constant punctate pressure or chemicals. The method is based on extracellular recordings of propagated action potentials from single-fiber receptive fields. This protocol details methods to identify and record from cutaneous primary afferent axons in an isolated mammalian skin–saphenous nerve preparation. ![]()
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